Korean J Ophthalmol > Volume 17(1); 2003 > Article
Korean Journal of Ophthalmology 2003;17(1):1-6.
DOI: https://doi.org/10.3341/kjo.2003.17.1.1    Published online June 30, 2003.
Effect of Nitric Oxide on the Proliferation of Cultured Porcine Trabecular Meshwork Cells.
Jae Woo Kim, Hyun Heo, Hyo Won Lee
Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea.
Abstract
To investigate the effect of nitric oxide (NO) on the proliferation of trabecular meshwork (TM) cells, primarily cultured porcine TM cells were exposed to NO donor (SNAP, -nitroso-N-acetyl-D, L-penicillamine) with and without its inhibitor (L-NAME, N (w) -Nitro-L-arginine methyl ester). The proliferation of TM cells was quantified by a rapid colorimetric assay. Acridine orange/Hoechest 33342 staining and flow cytometry with annexin-PI were done. As a result, NO inhibited the proliferation of TM cells significantly in a dose-dependent manner and this inhibitory effect was abolished by L-NAME. Fluorescent microscopy and flow cytometric analysis revealed that NO induced apoptotic cell death. The current results suggest that NO inhibit the proliferation of TM cells and apoptosis may be involved in some degree.
Key Words: apoptosis;nitric oxide;proliferation;trabecular meshwork cells


ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
SKY 1004 Building #701
50-1 Jungnim-ro, Jung-gu, Seoul 04508, Korea
Tel: +82-2-583-6520    Fax: +82-2-583-6521    E-mail: kos@ophthalmology.org                

Copyright © 2022 by Korean Ophthalmological Society.

Developed in M2PI

Close layer
prev next