Fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells.

Ahn,  Byung Heon; Oh,  Myung Ju; Bae,  Hyun Bong

doi:1987.1.2.90

Korean J Ophthalmol > Volume 1(2); 1987 > Article

Original Article

Korean Journal of Ophthalmology 1987;1(2):90-94.
DOI: https://doi.org/10.3341/kjo.1987.1.2.90 Published online December 30, 1987.

Fluorescent low density lipoprotein and acetylated low density lipoprotein labeling of cultured bovine trabecular endothelial cells.

Byung Heon Ahn, Myung Ju Oh, Hyun Bong Bae

Department of Ophthalmology, College of Medicine, Chungnam National University, Daejeon, Korea.

Abstract

A preliminary study was performed to investigate the staining characteristics of trabecula. endothelial cells with low density lipoprotein (LDL) and acetylated low density lipoprotein (Ac-LDL) labeled with a fluorescent probe, 1, 1`- dioctadecyl-3,3,3`, 3`- tetramethyl-indocarbocyanine perchlorate (Dil). Trabecular endothelial cells revealed a strong fluorescence with Dil-LDL, which was contradictory to the previous results obtained from other types of endothelial cells. These cells also showed a moderate fluorescence with Dil-Ac-LDL. Scleral fibroblasts and keratocytes showed a moderate to strong fluorescence with Dil-LDL and a weak fluorescence with Dil-Ac-LDL. Corneal endothelial cells revealed a very weak background fluorescence with Dil-LDL and a moderate fluorescence with Dil-Ar-LDL. Therefore, these four kinds of cells could not be definitely differentiated depending only on the staining characteristics with Dil-LDL and Dil-Ac-LDt.

Key Words: low density lipoprotein;acetylated low density lipoprotein;trabecular endothelial cell;tissue culture;Dil-LDL;Dil-Ac-LDL